Daily Practice Problems undefined Biotechnology: Principles and Processes

Question 1:

Polymerase chain reaction is technique in genetic engineering applied to: [Level: Moderate]

(a) induce protein synthesis

(b) amplify desired gene fragments

(d) visualise DNA fragments

Question 2:

Which of the following feature is not required in an ideal cloning vector? [Level: Moderate]

(a) Ori site

(b) Selectable marker

(d) Multiple recognition sites

Question 3:

DNA ligase in genetic engineering helps in: [Level: Moderate]

(a) joining DNA fragments

(b) addition of new nucleotides

(d) isolation of the genetic material

Question 4:

The first recombinant DNA was constructed by: [Level: Easy]

(a) Boyer and Smith

(b) Berg and Cohen

(d) Smith and Berg

Question 5:

Which of the following tools used in genetic engineering is referred to as ‘molecular scissor’? [Level: Easy]

(a) cloning vector

(b) restriction endonucleases

(d) probe

Question 6:

Select the incorrect statement. [Level: Difficult]

(a) Selection of recombinants due to inactivation of antibiotic is a cumbersome procedure.

(b) Insertional inactivation of β-galactosidase leads to colourless colonies.

(d) In insertional inactivation, the rDNA is inserted within the coding sequence of an enzyme

Question 7:

The thermostable DNA polymerase used during PCR is isolated from: [Level: Easy]

(a) Thermus aquaticus

(b) Bacillus amyloliquefaciens

(d) Thermus stuarti

Question 8:

The recognition sequence for restriction enzyme EcoRI is: [Level: Moderate]

(a) 5’ – GAATTC – 3’

(b) 5’ – GATTTC – 3’

(d) 5’ – GAATCC – 3’

Question 9:

In plasmid pBR322, what does BR represent? [Level: Moderate]

(a) bacterial strain

(b) name of the discoverers

(d) scientific name of the bacterium

Question 10:

The DNA molecule to which gene of interest is attached in order to generate its multiple identical copies is called: [Level: Moderate]

(a) template

(b) carrier

(d) multiplier

Question 11:

The first restriction endonuclease to be isolated was: [Level: Moderate]

(a) Hind II

(b) EcoR I

(d) Hind I

Question 12:

Read the following statements regarding gel electrophoresis: [Level: Difficult]

I. The DNA fragments are separated on agarose gel which is a natural polymer obtained from sea weeds.

II. During gel electrophoresis, DNA fragments move from anode towards cathode.

III. The larger fragment travels the largest distance over the agarose gel.

IV. To visualise DNA fragments, they are stained using ethidium bromide followed by exposure to UV light.

V. The extraction of DNA fragments from the agarose gel is called spooling.

In the light of above statements, identify the set of statements which are correct.

(a) I, II, III

(b) II, III, V

(d) I, IV only

Question 13:

From a mixture of biomolecules, DNA can be separated by treating it with: [Level: Moderate]

(a) ethanol at room temperature

(b) chilled methanol

(d) chilled chloroform

Question 14:

Which of the following vectorless gene transfer technique involves precipitation of recombinant DNA over the surface of tungsten or gold particles and shoot it into the target cells? [Level: Moderate]

(a) microinjection

(b) chemical mediated gene transfer

(d) Agrobacterium-mediated gene transfer

Question 15:

What will happen if a foreign gene is inserted in the ampicillin resistance gene of the vector pBR322? [Level: Difficult]
(a) The transformants will survive only in nutrient medium containing ampicillin.

(b) The transformants will survive only in nutrient medium containing tetracycline.

(d) The transformants will neither survive in ampicillin-containing medium nor in tetracycline-containing medium.

Question 16:

Statement I: [Level: Difficult]
EcoRI cuts the DNA between bases A and T only when the sequence GAATTC is present in the DNA.

Statement II:

EcoRI cuts slightly away from the centre of its recognition sequence and gives rise to sticky ends.

With respect to the statements given above, choose the correct answer from the options given below:

(a) Statement I is correct but Statement II is incorrect.

(b) Statement I is incorrect but Statement II is correct.

(d) Both Statement I and Statement II are correct.

Question 17:

Match the items given in Column I to Column II and find the correct answer from the options given below: [Level: Difficult]

Column I	Column II
1. BamHI	(i) Gene amplification
2. BAC	(ii) Vector
3. Yeast	(iii) Restriction endonuclease
4. PCR	(iv) Host organism

	1.	2.	3.	4.
(a)	(iii)	(ii)	(iv)	(i)
(b)	(ii)	(iii)	(iv)	(i)
(c)	(iii)	(ii)	(i)	(iv)
(d)	(ii)	(iii)	(iv)	(i)

Question 18:

Select the mismatched pair: [Level: Moderate]

(a) Cellulase – plant cell

(b) Lysozyme – bacterial cell

(d) Methylase – algal cell

Question 19:

The correct sequence of steps involved in PCR is: [Level: Easy]

(a) annealing, extension, denaturation

(b) denaturation, extension, annealing

(d) denaturation, annealing, extension

Question 20:

Which of the following steps is not associated with downstream processing? [Level: Easy]

(a) Separation

(b) Preservation

(d) Expression

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Application of Knowledge: Problem-solving allows you to apply the theoretical concepts of the topic NEET Biology Biotechnology: Principles and Processes you have learned to real-life situations. It helps you bridge the gap between abstract knowledge and practical scenarios, making the learning more relevant and meaningful.
Understanding Deeper Concepts: When you encounter problems related to a theoretical concept that you learned in NEET Biology Biotechnology: Principles and Processes NCERT Chapter 11, you are forced to delve deeper into its intricacies. This deeper understanding enhances your comprehension of the subject and strengthens your grasp of the underlying principles.
Critical Thinking: Problem-solving encourages critical thinking and analytical skills. It requires you to analyze the problem, identify relevant information, and devise a logical solution. This process sharpens your mind and improves your ability to approach complex challenges effectively.
Retention and Recall: Actively engaging in problem-solving reinforces your memory and improves long-term retention. Applying the concepts learned in Biotechnology: Principles and Processes NEET Biology in practical scenarios helps you remember them better than passive reading or memorization.
Identifying Knowledge Gaps: When you attempt to solve problems, you may encounter areas where your understanding is lacking. These knowledge gaps become evident during problem-solving, and you can then focus on filling those gaps through further study and practice. You can refer Biotechnology: Principles and Processes NEET Biology Notes on LearnoHub.com
Boosting Confidence: Successfully solving problems after learning a theoretical concept boosts your confidence in your abilities to handle Biotechnology: Principles and Processes. This confidence motivates you to tackle more challenging tasks and improves your overall performance in the subject.
Preparation for Exams and Challenges: Many exams, especially in science, mathematics, and engineering, involve problem-solving tasks. Regular practice in problem-solving prepares you to face these exams with confidence and perform well. It is also advised to take tests on Biotechnology: Principles and Processes NEET Biology Online Tests at LearnoHub.com.
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Life Skills Development: Problem-solving is a valuable life skill that extends beyond academics. It equips you with the ability to tackle various challenges you may encounter in personal and professional life.
Improving Decision Making: Problem-solving involves making decisions based on available information and logical reasoning. Practicing problem-solving enhances your decision-making skills, making you more effective in making informed choices.

In summary, problem-solving after learning a theoretical concept on CBSE Biotechnology: Principles and Processes NEET Biology is an essential part of the learning process. It enhances your understanding, critical thinking abilities, and retention of knowledge. Moreover, it equips you with valuable skills that are applicable in academic, personal, and professional contexts.

You must have heard of the phrase “Practice makes a man perfect”. Well, not just a man, practice indeed enhances perfection of every individual.

Practicing questions plays a pivotal role in achieving excellence in exams. Just as the adage goes, "Practice makes perfect," dedicating time to solve a diverse range of exam-related questions yields manifold benefits. Firstly, practicing questions allows students to familiarize themselves with the exam format and types of problems they might encounter. This familiarity instills confidence, reducing anxiety and improving performance on the actual exam day. Secondly, continuous practice sharpens problem-solving skills and enhances critical thinking, enabling students to approach complex problems with clarity and efficiency. Thirdly, it aids in identifying weak areas, allowing students to focus their efforts on improving specific topics. Moreover, practice aids in memory retention, as active engagement with the material reinforces learning. Regular practice also hones time management skills, ensuring that students can allocate appropriate time to each question during the exam. Overall, practicing questions not only boosts exam performance but also instills a deeper understanding of the subject matter, fostering a holistic and effective learning experience.

All About Daily Practice Problems on NEET Biology Biotechnology: Principles and Processes NCERT Chapter 11

Our Daily Practice Problems (DPPs) offer a diverse range of question types, including Multiple Choice Questions (MCQs) as well as short and long answer types. These questions are categorized into Easy, Moderate, and Difficult levels, allowing students to gradually progress and challenge themselves accordingly. Additionally, comprehensive solutions are provided for each question, available for download in PDF format - Download pdf solutions as well as Download pdf Questions. This approach fosters a holistic learning experience, catering to different learning styles, promoting self-assessment, and improving problem-solving skills. With our well-structured DPPs, students can excel in exams while gaining a deeper understanding of the subject matter. Hope you found the content on NEET Biology Biotechnology: Principles and Processes NCERT Chapter 11 useful.

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