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Question:
An angular magnification (magnifying power) of 30X is desired using an objective of focal length 1.25cm and an eyepiece of focal length 5cm. How will you set up the compound microscope?
Answer:

Focal length of the objective lense (f0bj) = 1.25 cm

Focal length of the eyepiece (feye) = 5 cm

Distance of vision (d) = 25 cm

Angular magnification of the compound microscope = 30X

Total magnification power of the compound microscope (m)= 30

Angular magnification of the eyepiece

meye = (1+d/feye) = (1+25/5) = 6

Angular magnification of the object (mobj) is related to meye

Thus, mobj = m/ meye = 30/6 = 5

Also, mobj = image distance for the objective lens (vobj)/ Objective distance for the objective lens (-uobj)

5 = vobj /-uobj

vobj  = -uobj x 5                                      (i)

Using lens formula

1/ vobj  - 1/ uobj = 1/fobj

1/1.25= 1/-5 uobj - 1/ uobj

1/1.25= -6/ uobj

uobj = -6/5 x 1.25 = -1.5 cm

vobj = -5 uobj = -5 x (-1.5) = 7.5 cm

The object should be placed 1.5 cm away from the objective lens.

Using lens formula

1/ veye  - 1/ ueye = 1/feye

Where,  veye  = image distance for the eyepiece = -d = -25 cm

ueye = Object distance for the eyepiece

1/ ueye = 1/ veye  -  1/feye = -1/25 - 1/5 = -6/25

ueye = 4.17 cm

Separation between the objective lens and eyepiece = ueye + vobj = 4.17 + 7.5 = 11.67 cm

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