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Question:
mam in gene gun method after shooting the dna in nucleus ,what happens with the tungsten atom used in the process, does it remain in the cell and if not then what happens to it
Answer:

In gene gun method, tungsten or gold called microprojectiles coated with DNA to be used for transformation. They are accelerated to velocity and enable their entry into plant cells or nuclei.

Tungsten particles are cheaper but are irregular shape and size, may be toxic to certain cells types and show surface oxidation, precipitation of DNA or form aggregates and reduces dispersion.

But gold particles are uniform in size with lower toxicity so. More preferable.

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