Question:
Hello. Why does the vector DNA after ligating the foreign DNA produce tranformants with non recombinant vector? (Part 14 video)
Answer:
The part of the foreign DNA that after ligation with the vector DNA becomes a part of the host DNA itself, or the host system recognises the foreign DNA integrated into the vector DNA as of self. Thus, all the expression genes are expressed in the host, even the ones which belong to the foreign DNA.
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